Mechanical stiffness of reconstituted actin patches correlates tightly with endocytosis efficiency
نویسندگان
چکیده
منابع مشابه
Yeast actin patches are networks of branched actin filaments
Cortical actin patches are the most prominent actin structure in budding and fission yeast. Patches assemble, move, and disassemble rapidly. We investigated the mechanisms underlying patch actin assembly and motility by studying actin filament ultrastructure within a patch. Actin patches were partially purified from Saccharomyces cerevisiae and examined by negative-stain electron microscopy (EM...
متن کاملActin Filament Severing by Cofilin Dismantles Actin Patches and Produces Mother Filaments for New Patches
BACKGROUND Yeast cells depend on Arp2/3 complex to assemble actin filaments at sites of endocytosis, but the source of the initial filaments required to activate Arp2/3 complex is not known. RESULTS We tested the proposal that cofilin severs actin filaments during endocytosis in fission yeast cells using a mutant cofilin defective in severing. We used quantitative fluorescence microscopy to t...
متن کاملMovement of cortical actin patches in yeast
In yeast, actin forms patches associated with the plasma membrane. Patch distribution correlates with polarized growth during the cell cycle and in response to external stimuli. Using green fluorescent protein fused to capping protein to image actin patches in living cells, we find that patches move rapidly and over long distances. Even patches in clusters, such as at the incipient bud site, sh...
متن کاملSymmetry breaking in reconstituted actin cortices
The actin cortex plays a pivotal role in cell division, in generating and maintaining cell polarity and in motility. In all these contexts, the cortical network has to break symmetry to generate polar cytoskeletal dynamics. Despite extensive research, the mechanisms responsible for regulating cortical dynamics in vivo and inducing symmetry breaking are still unclear. Here we introduce a reconst...
متن کاملUpdate on Endocytosis Endocytosis, Actin Cytoskeleton, and Signaling
Endocytosis is the internalization of plasma membrane proteins and lipids, extracellular molecules, fluids, particles, exosomes, viruses, and bacteria. Endocytic internalization is a conserved process for all eukaryotic cells that is required for diverse cellular functions. These include turnover and degradation of plasma membrane proteins and receptors, transduction and dispersal of signals wi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: PLOS Biology
سال: 2019
ISSN: 1545-7885
DOI: 10.1371/journal.pbio.3000500